High-resolution crystal structure and biochemical characterization of a GH11 endoxylanase from Nectria haematococca
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Recombinant expression, purification and biochemical characterization of kievitone hydratase from Nectria haematococca
Kievitone hydratase catalyzes the addition of water to the double bond of the prenyl moiety of plant isoflavonoid kievitone and, thereby, forms the tertiary alcohol hydroxy-kievitone. In nature, this conversion is associated with a defense mechanism of fungal pathogens against phytoalexins generated by host plants after infection. As of today, a gene sequence coding for kievitone hydratase acti...
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A previous study reported the isolation of two mutants (LE1 and LE2) of the plant pathogenic fungus Nectria haematococca (anamorph, Fusarium solani f. sp. cucurbitae) with macroconidia with reduced ability to adhere (Att) to zucchini fruits and polystyrene. The adhesion-reduced-phenotype in LE1 and LE2 macroconidia is temperature sensitive and dependent on the concentration of nutrients. Classi...
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We identified a polyketide synthase (PKS) gene, pksN, from a strain of Nectria haematococca by complementing a mutant unable to synthesize a red perithecial pigment. pksN encodes a 2,106-amino-acid polypeptide with conserved motifs characteristic of type I PKS enzymatic domains: beta-ketoacyl synthase, acyltransferase, duplicated acyl carrier proteins, and thioesterase. The pksN product groups ...
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UNLABELLED During the last decade the use of transaminases for the production of pharmaceutical and fine chemical intermediates has attracted a great deal of attention. Transaminases are versatile biocatalysts for the efficient production of amine intermediates and many have (S)-enantiospecificity. Transaminases with (R)-specificity are needed to expand the applications of these enzymes in bioc...
متن کاملHigh-Resolution Crystal Structure of RpoS Fragment
Legionella pneumophila RpoS (LpRpoS) is an alternative sigma factor of RNA polymerase 13 (RNAP) essential for virulence and stress resistance. To investigate the mechanism of RpoS in the 14 intracellular pathogen L. pneumophila, we determined the high-resolution crystal structure of the 15 LpRpoS (residues 95-194) containing a partial region 1.2 and region 2. The structure of LpRpoS 16 (residue...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2020
ISSN: 2045-2322
DOI: 10.1038/s41598-020-72644-w